携带blaCTX-M-65、rmtB和fosA3多重耐药质粒pHN7A8的适应性研究

doi:10.11843/j.issn.0366-6964.2016.01.023

Abstract

Abstract:

The objective of this study was to examine the fitness cost of a multidrug plasmid pHN7A8 carrying bla_CTX-M-65，rmtB，and fosA3.Plasmid pHN7A8 carrying bla_CTX-M-65，rmtB，and fosA3 from E.coli 7A8 was transferred into E.coli JM109 by electroporation.Transformant 7A8JT containing pHN7A8 was obtained.Growth curve and growth competition experiments were performed to assess the fitness cost of pHN7A8 upon its host.Growth curve showed no significant difference between the transformant carrying pHN7A8 and sensitive recipient E.coli JM109.Competition experiments between E.coli JM109 strain and transformant 7A8JT revealed a competitive advantage for the transformant versus the recipient.The results showed that no fitness cost was observed for plasmid pHN7A8.More attention should be taken into account the effect of the clinical use of antibiotics on the selection of multidrug resistance encoding plasmids.

CLC Number:

S852.612

HE Liang-ying，LIU Lan-ping，ZENG Zhen-ling，LIU Jian-hua. Fitness Cost of a Multidrug Plasmid Carrying bla_CTX-M-65，rmtB，and fosA3[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 172-176.

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References

［1］YU F Y，YAO D，PAN J Y，et al.High prevalence of plasmid-mediated 16S rRNA methylase gene rmtB among Escherichia coli clinical isolates from a Chinese teaching hospital［J/OL］.BMC Infect Dis，2010，10：184.［2015-9-18］.http：//www.biomedcentral.com/1471-2334/10/184.
［2］XIA L N，TAO X Q，SHEN J Z，et al.A survey of β-lactamase and 16S rRNA methylase genes among fluoroquinolone-resistant Escherichia coli isolates and their horizontal transmission in Shandong，China［J］.Foodborne Pathog Dis，2011，8（12）：1241-1248.
［3］DU X D，WU C M，LIU H B，et al.Plasmid-mediated ArmA and RmtB 16S rRNA methylases in Escherichia coli isolated from chickens［J］.J Antimicrob Chemother，2009，64（6）：1328-1330.
［4］DENG Y，HE L，CHEN S，et al.F33：A-：B- and F2：A-：B- plasmids mediate dissemination of rmtB-blaCTX-M-9 group genes and rmtB-qepA in Enterobacteriaceae isolates from pets in China［J］.Antimicrob Agents Chemother，2011，55（10）：4926-4929.
［5］YAO Q，ZENG Z，HOU J，et al.Dissemination of the rmtB gene carried on IncF and IncN plasmids among Enterobacteriaceae in a pig farm and its environment［J］.J Antimicrob Chemother，2011，66（11）：2475-2479.
［6］HOU J，HUANG X，DENG Y，et al.Dissemination of the fosfomycin resistance gene fosA3 with CTX-M β-lactamase genes and rmtB carried on IncFII plasmids among Escherichia coli isolates from pets in China［J］.Antimicrob Agents Chemother，2012，56（4）：2135-2138.
［7］HE L，PARTRIDGE S R，YANG X，et al.Complete nucleotide sequence of pHN7A8，an F33：A-：B- type epidemic plasmid carrying blaCTX-M-65，fosA3 and rmtB from China［J］.J Antimicrob Chemother，2013，68（1）：46-50.
［8］ZHANG Q，LIN J，PEREIRA S.Fluoroquinolone-resistant Campylobacter in animal reservoirs：dynamics of development，resistance mechanisms and ecological fitness［J］.Anim Health Res Rev，2003，4（2）：63-71.
［9］JOHNSEN P J，SIMONSEN G S，OLSVIK O，et al.Stability，persistence，and evolution of plasmid-encoded VanA glycopeptide resistance in enterococci in the absence of antibiotic selection in vitro and in gnotobiotic mice［J］.Microb Drug Resist，2002，8（3）：161-170.
［10］FOUCAULT M L，COURVALIN P，GRILLOT-COURVALIN C.Fitness cost of VanA-type vancomycin resistance in methicillin-resistant Staphylococcus aureus［J］.Antimicrob Agents Chemother，2009，53（6）：2354-2359.
［11］宁官保，牛艺儒，张鼎，等.鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究［J］.畜牧兽医学报，2015，46（6）：1018-1025.
NING G B,NIU Y R,ZHANG D,et al.Studies on the drug resistance of chiken isolated Escherichia coli and role of traditional Chinese mediciues in drug resistance elimination［J］.Acta Veterinaria et Zootechnica Sinica,2015,46(6):1018-1025.(in Chinese)

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Fitness Cost of a Multidrug Plasmid Carrying bla_CTX-M-65，rmtB，and fosA3

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Fitness Cost of a Multidrug Plasmid Carrying blaCTX-M-65，rmtB，and fosA3

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Fitness Cost of a Multidrug Plasmid Carrying bla_CTX-M-65，rmtB，and fosA3